Nuban Institute

Different Methods for DNA Purification

DNA purification is one of the most commonly used and crucial methods in molecular biology. Purification of DNA aims at getting rid of the desired genetic material (chromosomal material) from other contaminants like proteins as well as RNA and cell membrane. This is a vital step in virtually all molecular applications and must be performed correctly in order to obtain top-quality, usable DNA.

There are a variety of options for DNA purification. The selection is based on a variety of variables like the source materials and downstream applications, cost, and time constraints. Common DNA purification methods include chemical treatment, enzymatic digestion, or mechanical disintegration of samples of cells or tissues followed by salting-out the proteins and precipitation of the DNA with alcohol.

Ethanol precipitation is a cheap simple, quick and easy method of desalting and concentrating DNA. DNA molecules are aggregated in the presence monovalent cations such as sodium, and then are removed from the solution by using high concentrations of ethanol. This technique is used to remove organic compounds, and other impurities. It is usually employed with other purification methods.

Another popular method for DNA purification is anion exchange chromatography. DNA in solution is linked to positively charged resins through the interaction more information between the negatively charged DNA phosphate backbone and the positively charged surface molecules of the resin. During the binding steps the contaminants are removed the use of a rigorous washing process. The DNA purified is eluted using low-salt conditions.

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